Shout out to former postdoc, Will Harcombe, on bring promoted to Associate Professor this past summer. Very exciting to see how well things have gone with his lab’s exploration of the evolution of cooperation.
Deepa Agashe, at NCBS in Bangalore, has been awarded one of three “SERB Women Excellence” Awards and was recognized directly by the president. Not that president, but the Indian President, Shri Ram Nath Kovind. As they call out on their website:
Dr Deepa Agashe is a biologist, working at the interface of evolutionary biology, ecology, and molecular biology. Using diverse tools such as experimental evolution, genomics, molecular analyses and phylogenetics, she aims to understand how bacteria and insects adapt to new environments. Dr. Agashe received her Bachelor’s degree in Microbiology from Abasaheb Garware College (University of Pune) in 2003, followed by a PhD from the University of Texas at Austin, the USA in 2009. Her thesis work showed that genetic diversity could stabilize animal populations and facilitate adaptation to new niches. During her postdoctoral work at Harvard University, USA, she proved that so-called “silent” mutations could have enormous effects on bacterial evolution. In 2012, she returned to India to lead a research group at the National Centre for Biological Sciences (NCBS–TIFR), where her team is dedicated to unravelling the causes and mechanisms of evolution.
Way to go Deepa! Yet another lab alum making us all proud.
Congrats to Dipti Nayak, who is still in her first year of being an Assistant Professor in Molecular and Cellular Biology at UC Berkeley, on being named one of the 15 Searle Scholars for 2020. She was honored due to exploring “how do archaea produce and consume the greenhouse gas methane, and how can they be engineered to address environmental and biotechnological challenges.” You make us all proud, Dipti!
Remember March 1st of this year? Kids were in school and labs were open. As for me, I was about to get on a plane to fly from Copenhagen through to Denver for the American Physical Society (APS) general meeting to give a talk. With less than 24 hours to go before the meeting began the whole >10K person meeting was cancelled. This was, of course, just the beginning of many changes that were to come.
APS has decided to open up the ability for presenters to post slides of their talks and I have gone ahead and put my slides up online. This is basically the abbreviated version of Jessica and Siavash’s paper that I mentioned below. I was sorry to miss the opportunity to see people there, but I look forward to attending to present in the same session next year in Nashville. Let’s hope that, by then, social distancing will be a distant memory from the past.
Yup, I really wrote that. In a lab so strongly influenced by Darwin, I just invoked the name of Lamarck, who is often framed as the anti-Darwin. In no way am I defending any aspect of Lamarck’s support of the “Great chain of being” to describe the
(super)natural progression of organisms through time. Rather, I am invoking the evolution based upon acquired phenotypes. This has been rightly dismissed as being able to describe any broad timescale of evolution, but what about the potential role for epigenetics? Might anything here be considered evolution?
Late in 2019, a paper from our lab championed by Jessica Lee and co-first author Siavash Riazi describes how Jessica discovered that phenotypic variation can permit a population of Methylobacterium extorquens to survive an otherwise lethal formaldehyde stress (PLOS Genetics, 2019. 15:e1008458).
Jessica found that the rare survivors were phenotypic variants (no mutations anywhere), supported by several methods including excellent video microscopy data (below) from Shahla Nemati in the lab of Andreas Vasdekis here at University of Idaho (who recently was promoted to Associate Professor in Physics, woo hoo!). Rather than having a population of discrete populations of highly tolerant and weakly tolerant cells (akin to antibiotic persistence), Jessica found that the concentration that cells could survive was a wide, dynamic continuum.
Siavash led our effort to model how this distribution of tolerance changes in different environments in terms of growth, death, and transitioning across a continuous scale of formaldehyde tolerance. Working with co-advisor Chris Remien, Ben Ridenhour, Jessica, and myself, he was able to develop a simple PDE that allows us to conclude, amongst other things, that formaldehyde tolerance is inherited over a large number of generations. Finally, Jessica and Jannell Bazurto analyzed RNA-Seq data to determine that these phenotypically tolerant cells have a distinct transcriptome from either low tolerant cells or the immediate stress response of low tolerance cells to formaldehyde exposure.
All told, we observe selection upon phenotypes that show a fair amount of phenotypic inheritance: all the ingredients that define evolution. No allele changes were observed, but the distribution of inherited phenotypes did change. As a visual metaphor, I discussed this scenario with visual artist Cody Muir, who was at IBEST, and this resulted in a fun visual that mixes seeing only some colonies grow with the Lamarckian metaphor of the giraffe’s neck:
Moving forward, we look forward to uncovering the physiological basis of phenotypic heterogeneity/inheritance, as well as beginning to look at how Lamarckian and Darwinian processes can jointly influence evolutionary outcomes.
It has been a pleasure to bring some new faces into the lab over the past months. Olivia Speare – now Olivia Benski – has transitioned from an undergrad to a technician working on our EPSCoR project with Tomislav. Monica Pedroni has joined us a second lab technician, and has been working with Sergey on both our NSF Dimensions of Biodiversity project and our DOE Biosystems one. Cole Garrett and Drew Johnson are both doing undergrad research in the lab, Cole with Sergey looking at PHB accumulation and Drew with Tomislav looking at evolution of growth on methoxylated aromatics. Giovanna Girolami has joined us as an UG tech making media, doing sterilization, etc. And since the last such post, we have had Mete Yuksel, Hannah Ringel, and Maria Elizarraras join the lab and then move on to other opportunities.
This summer Dipti Nayak began her Assistant Professor position in Molecular and Cellular Biology at UC Berkeley. I am very excited for her, and was thrilled to visit her new space last month when in the area. She brings the total number of PIs from the lab to ten… Best wishes to her!
In the morass of metagenomic sequence data on microbes, it is unclear what a unit should even be in a community. Ideally, one would want to uncover the core populations present: the set of strains that actually are exchanging genetic information and are reasonably cohesive, even in the presence of a degree of input from outside sources. The Polz lab has put forward a fantastic new method in Arevalo et al. “A reverse ecology approach based on a biological definition of microbial populations” (Cell, 2019. 178:820-834). for doing so that is based upon the simple concept that the length distribution of perfectly matching sequences can serve as a chronometer of separation. In the absence of exchange, two lineages emerging from the same genome sequence will ultimately accumulate random differences that will leave behind an exponential distribution of perfect sequence matches. If they continue to exchange, however, this will lead to an excess of longer sequence matches than expected. Indeed, this pattern is seen amongst strains previously identified as being of the same population. Furthermore, there is the substantial advantage that it identifies precisely which genes are being exchanged, thereby providing clues as to the success of one population versus each other, hence the concept of a “reverse” approach to ecology.
In our Preview – “Align to define: ecologically meaningful populations from genomes” (Cell, 2019. 178:767-768) – Sergey Stolyar, a Research Associate Professor working with my group, and I point out how these units defined upon exchange are actually narrower than “exact sequence variants” (ESVs), which have already been thought of as too narrow by some. We point out that the ecological distinctiveness of lineages in lab-based experimental evolution is often but one or a few mutations, so this seems quite consonant with their findings. It remains unclear whether these populations are equivalent to “species,” but this represents an exciting and refreshingly novel approach to letting genome sequences tell us what we should be paying attention to.
None of us are looking for extra work to do, but when invited to write a Perspective for this great paper out of the lab of Andreas Wagner, Jessica Lee (now at Global Viral) and I made time to make it happen. The Research Article from Zheng et al. – “Cryptic genetic variation accelerates evolution by opening access to diverse adaptive peaks” (Science, 2019. 365:347-353) – took advantage of the ability to select upon fluorescent proteins in a FACS machine to impose various selective pressures. They permitted cryptic variation to accumulate by imposing purifying selection upon yellow fluorescence for yfp variants. They then used this pool to begin selecting upon the upper range of green fluorescence. These diverse pools adapted faster and through a wider variety of paths than if they just began with the single ancestral yfp variant.
In our Perspective – “Tales from the cryp(ic)” (Science, 2019. 365:318-319) – we enjoyed pointing out comparisons to some awesome work of friends and colleagues that touched upon these questions: Jeremey Draghi‘s exploration of robustness, Eric Hayden‘s similar work with ribozymes, and Joe Thornton and Mike Harm‘s use of ancestral reconstruction to look at epistasis from a historical perspective.