People

People and Contact Information

 


Principal Investigator

img_7304Christopher J. Marx
Professor of Biology
University of Idaho | Department of Biological Sciences
Institute for Bioinformatics and Evolutionary Studies
Center for Modeling Complex Interactions
Gibb 134 | 875 Perimeter Drive MS 3051 | Moscow, ID 83844-3051 cmarx@uidaho.edu

 

*Photo is with Rasmus and Solvei in Jasper National Park.


 Research Associate Professor

 

screen-shot-2016-11-28-at-4-36-53-pm

Sergey Stolyar

sstolyar (at) uidaho.edu

I knew Chris from our time together in Mary Lidstrom’s lab at UW. Since then, I have had a series of research positions at the Institute for Systems Biology, UW, and PNNL. I have been happy to join the team, and have focused my efforts on our DOE project studying synthetic microbial communities, and upon using a combination of engineering and evolution to bring novel metabolic capacities to Methylobacterium.

 


Postdoctoral Fellows

 

Lon Chubiz

lchubiz (at) fas.harvard.edu

My focus in the Marx Lab is in understanding the genetic and physiological basis underlying adaption. Particularly, how does underlying pleiotropy and epistasis constrain the adaptive trajectories available to an organism challenged with a new environment? Cellular metabolism provides an ideal framework to address this question. Using the aerobic methylotrophMethylobacterium extorquens as model system allows this question to be approached from the perspective of metabolic specialization. By understanding the mechanistic basis for observed phenotypes in evolved populations, I believe answering this question is possible.

 Sean Michael Carroll

scarroll (at) oeb.harvard.edu

My work explores how various mechanisms of adaptation lead to improved growth in Methylobacterium extorquens AM1, a model microbe for studies of one-carbon (C1) metabolism and microbial evolution. I study at a systems-level the mechanisms by which strains acclimate and adapt to an engineered C1 metabolism: using whole-genome sequencing, microarray analyses, and quantification of metabolites to connect genotypes to higher-order properties such as growth rate and fitness. In a complementary approach, I use a regulated promoter system to directly modulate the activity of key C1 enzymes and measure the effect of these “idealized mutations” on growth rate and fitness. And with the help of students from this year’s OEB100 (2012) class, I am documenting the genomic and phenotypic changes that our model microbe has undergone during fifty years of life in the lab. Together, I hope to help illuminate how factors such as epistasis, pleiotropy, and clonal interference influence adaptive trajectories and shape microbial functions in the lab, in the wild, or in human health and disease.

William Harcombe

wharcombe (at) oeb.harvard.edu

Microbes are capable of incredible metabolic feats, acquiring energy from a diverse array of compounds. I am interested in how evolution shapes these metabolic capabilities. At the single species level I study how bacteria adapt to acquisition of a novel metabolic pathway. At the multi-species level I study how adaptation alters the productivity of model consortia. Metabolism of various compounds often depends on a complex network of interacting bacteria. I use model microbial assemblages (combinations of E. coli,Salmonella, and Methylobacterium) to test how ecological and evolutionary processes influence community composition and function.

Joshua Michener

michener(at)fas.harvard.edu

I study adaptation following horizontal gene transfer, seeking to understand the co-evolutionary process as a pathway adapts to its new host and a host to its new pathway. Specifically, I’m looking at the growth of Methylobacterium extorquens on a toxic pollutant, dichloromethane. A pathway for dichloromethane utilization exists in one strain of M. extorquens, but does not function when transferred to a closely related strain. I’m using experimental evolution to evolve the pathway in its new host, exploring the adaptive mechanisms and trajectories.


Graduate Students

 

Dipti Nayak

dnayak (at) oeb.harvard.edu

My research focuses on using experimental evolution to understand microbial physiology. For my PhD I am trying  to understand the physiological basis of formaldehyde stress response as well as the genetics of the N-methyl glutamate pathway for methylamine utilization in Methylobacterium extorquens using experimental evolution.

 

José Rojas Echenique

rojasechenique@fas.harvard.edu

I’m interested in the possible forms that the organisms in a population can take on as they evolve. Conceptually we organize these forms according to their fitness and mutational distance from one another on a fitness landscape. I’m working towards an understanding of the physiological traits and interactions that make up a model fitness landscape: the central carbon metabolism of Methylobacterium extorquens.

http://www.people.fas.harvard.edu/~rojasechenique/

Sarah Douglas

 sdouglas@mcb.harvard.edu

“Costly cooperation between unrelated organisms presents a Darwinian dilemma: if natural selection favors individuals with the highest relative fitness, why should an organism increase the fitness of its neighbors? Using a unique bacterial system that allows the de novo evolution of cooperation, I am characterizing the ecological dynamics and molecular mechanisms underlying the rise of this social behavior. I also study how selection sorts variations in cooperative strategies within a population, and how this variation affects resistance to cheater invasion. Knowing how mutational diversity impacts cooperative phenotypes, and how selection acts upon phenotypic variety, will lead to a more accurate understanding of dynamic, ecologically complex systems of cooperation.”

 

Nicholas Leiby

leiby (at) fas.harvard.edu

I’m a PhD student in Systems Biology.  I study E. coli evolving over long periods in laboratory conditions, and the tradeoffs that occur in metabolic flexibility in order to optimize for the present environment.

 

 


Former Lab Members

 

Paige Swanson – former lab manager

 

As a research technician and lab manager, I worked with postdoc Alex Bradley and graduate student Nigel Delaney to answer questions about Methylobacterium populations that were evolved experimentally in our lab. I am a transplant from Colorado, where I studied Evolutionary Biology at the University of Colorado at Boulder. When not in the lab, you’ll typically find me in the kitchen. I am now a Director at Finch Therapeutics in Somerville, MA.

 

Chris on Rumble Seat pin up shoot

Chris Engles – former lab assistant

When I wasn’t sterilizing glassware, ordering lab supplies or updating WordPress sites, I’m taking pictures or shooting movies.  On weekends I can typically be found at my satellite office.

 

Former Postdocs:

Alexander S. Bradley (2008-12, now Assistant Professor, Earth and Planetary Sciences, Washington U., MO)  http://bradleylab.wustl.edu

Jessica Purswani (2011-12, now postdoctoral fellow, U. Granada, Spain)

Deepa Agashe (2008-11, now Fellow (PI), Ecology and Evolution, National Centre for Biological Sciences, Bangalore, India) http://ncbs.res.in/dagashe

Completed Ph.D. theses:

Nigel F. Delaney (2012, now postdoctoral fellow, Broad Institute, Cambridge, MA)

Ming-Chun “Miki” Lee (2010, now postdoctoral fellow, Biochemistry, U. Hong Kong)

Hsin-Hung “David” Chou (2009, now an EMBO postdoctoral fellow, Inst. Of Molecular Systems Biology, ETH, Zürich)

Visiting Erasmus Mundus M.S. thesis:

Alex Betts (2012-2013, future Ph.D. student, Oxford University)

Özden Baltekin (2011-12, now Ph.D. student, Uppsala U., Sweden)

Completed B.A. theses:

Lewis M. Ward (2010, future Ph.D. student, Geological and Planetary Sciences, CalTech, CA)

David G. Robinson (2009, now Ph.D. student, Quantitative and Computational Biology, Princeton, NJ)

Other former rotation/collaborating graduate students: Zain Ali, Anthony Blair, Hsiao-Han Chang, Delbert “Andre” Green, Arnon Levy, Tami Lieberman, Niall Mangan, Jue Wang

Other former undergraduates: Earl “Alex” Pickett, Chris Ding, Dan Lorenzana, Sherif Gerges, David Guernsey, Ed Kogan, Jessica Berger, Julia Berthet, Kiahana Brooks, Lela Sims, Lucy Liu, Roxana Tarnita, Samantha Parker, Vicky Wu, Will Polachek

 

Former high school student: Jaya Gandhi

 

Former technicians: Anthony Blair, Maggie Chen, Melissa Derby, Maryska Kaczmarek(now Ph.D. student, Integrative Biology, U. Texas, Austin)

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